Stabilizing labile DNA-protein complexes in polyacrylamide gels
نویسندگان
چکیده
منابع مشابه
Comparison of various ultraviolet sources for fluorescent detection of ethidium bromide-DNA complexes in polyacrylamide gels.
Ultraviolet sources with output wavelengths or 254, 300, and 366 nm were compared for detection of ethidium bromide-DNA complexes in acrylamide gels. The 254and 300-nni sources were both much more sensitive than the 366-nni source. The 254-nni .source produced a great deal of photodamage, photonickingand photodimerization, and photobleaching, while the longer wavelength sources cause little dam...
متن کاملDetection of DNA in polyacrylamide gels by autoradiography.
METHOD 1. Immerse the gel, together with its attached glass plate, in 7% acetic acid for 5 minutes. Remove the gel from the fixative by carefully lifting the glass plate from the fluid. 2. Rinse the gel briefly in deionized H2O. Remove excess fluid from the surface of the gel with a pad of Kimwipes. IMPORTANT Do not use absorbent paper; it will stick to the gel. 3. (Optional) Dry the gel onto a...
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A new method for on-chip sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of proteins is reported. Miniaturization of SDS-PAGE has attracted significant attention because it offers rapid analysis times, excellent resolution, high throughput, and the potential for integration and automation, as compared to conventional counterparts. The presented on-chip SDS-PAGE technique em...
متن کاملPhotopatterned free-standing polyacrylamide gels for microfluidic protein electrophoresis.
Designed for compatibility with slab-gel polyacrylamide gel electrophoresis (PAGE) reagents and instruments, we detail development of free-standing polyacrylamide gel (fsPAG) microstructures supporting electrophoretic performance rivalling that of microfluidic platforms. For the protein electrophoresis study described here, fsPAGE lanes are comprised of a sample reservoir and contiguous separat...
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ژورنال
عنوان ژورنال: ELECTROPHORESIS
سال: 2010
ISSN: 0173-0835,1522-2683
DOI: 10.1002/elps.200900573